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A preliminary study on the freezing tolerance of milkfish (Chanos chanos, Forskal) embryos

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dc.contributor.advisorGeduspan, Jane S.
dc.contributor.authorBuendia, Jeremiah C.
dc.contributor.chairTorres, James L.
dc.contributor.committeememberEmata, Arnil
dc.contributor.committeememberFormcion, Minda
dc.date.accessioned2026-07-21T02:26:56Z
dc.date.issued1994-03
dc.description.abstractMilkfish embryos of blastula and C-shaped stages were tested for cryoprotectant toxicity using DMSO and glycerol at 10%, 12% and 15% concentrations. Survival and hatching as well as the effects of freezing were observed. Glycerol was more toxic than DMSO. Freezing at 4°C resulted in the decrease of hatching, and ice crystal formation killed the embryos during the process of freezing to -80°C. C-shaped embryos had a higher survival and hatching than the blastula stage embryos in the toxicity and cold shock tests.
dc.format.extentviii, 37 p.
dc.identifier.citationBuendia, J. C. (1994). <i>A preliminary study on the freezing tolerance of milkfish (Chanos chanos, Forskal) embryos</i> [Undergraduate thesis, University of the Philippines Visayas]. UPV Institutional Repository. https://hdl.handle.net/20.500.14583/761
dc.identifier.urihttps://hdl.handle.net/20.500.14583/761
dc.publisherDivision of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas
dc.subjectMilkfish
dc.subjectFish embryos
dc.subjectCryopreservation
dc.subjectDimethyl sulfoxide (DMSO)
dc.subjectAquaculture
dc.subject.agrovocmilkfish
dc.subject.agrovocanimal embryos
dc.subject.agrovoccold tolerance
dc.subject.agrovocegg hatchability
dc.subject.agrovocaquaculture
dc.subject.lccLG 993.5 1994 B5 B94
dc.subject.sdgSDG 2 - Zero hunger
dc.subject.sdgSDG 9 - Industry, innovation and infrastructure
dc.subject.sdgSDG 14 - Life below water
dc.titleA preliminary study on the freezing tolerance of milkfish (<i>Chanos chanos</i>, Forskal) embryos
dc.typeThesis
thesis.degree.departmentCollege of Arts and Sciences
thesis.degree.grantorUniversity of the Philippines Visayas
thesis.degree.levelUndergraduate
thesis.degree.nameBachelor of Science in Biology

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