Algal organic matter fluorescence analysis of <i>Chlorella</i> sp. for biomass estimation

Abstract

Algal Organic Matter (AOM) is derived from the dissolved organic matter composition of the algal species being observed. In this study, excitation–emission fluorescence spectroscopy was used to determine Chlorella sp.’s AOM and pigment characteristics in varying algal biomass concentrations. The AOM and pigment characteristics were observed at 400–600 nm and 600–800 nm fluorescence emission, respectively, with an excitation spectrum of 300–450 nm. F450/680 was computed based on the ratio between the dissolved organic matter contribution at 450 nm and chlorophyll-a at 680 nm. F450/680 positively correlated with algal biomass (r = 0.96) at an excitation wavelength of 405 nm. This study is a good reference for those interested in algal biomass estimation and production in natural waters.