Undergraduate Special Problem
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Item Bacteriological survey and detection of Escherichia coli, Salmonella spp., Streptococcus spp., and Vibrio spp. in bottom sediments from two sites in Iloilo RiverBarrera, Christopher (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-04)Bacterial contamination in the Iloilo river occurs because of various human activities. The assessment of the river was done using microbiological parameters (HPC, TC, FC, and FS) for 7 months in Sooc Bridge and Carpenter’s Bridge using bottom sediments for samples. Sediment was used because bacteria accumulate over time in the sediment due to high amounts of organic and inorganic matter in the substrate and this leads to better survivability of bacteria. The values obtained from microbiological parameters investigated are generally higher in Sooc Bridge. HPC results show that the higher mean value was in Sooc Bridge(7.43 x 108 CFU/lOOg). The levels of TC on both sampling sites ranged from 9.5 x 102to 2.40 x 104 CFU/100g. For TC, Sooc has the higher mean (1.76 x 104 CFU/lOOg) for 7 months. FC values ranged from 3.0 x 101 to 2.4 x 104 CFU/lOOg. Sooc bridge has the higher mean(1.17 x 104 CFU/lOOg) between the 2 sites. Lastly, the FS value range from 2.9 x 103 to 2.4 x 104 CFU/lOOg. Also, Sooc Bridge has the higher mean value (1.58 x 104 CFU/lOOg). There are no significant differences on the microbiological parameters used in each site. The FC/FS ratio revealed that mixed fecal materials contaminate the river. Among the four pathogenic bacteria detected, Salmonella spp. was the most abundant for 7 months (63-65%) and E. coli was the least abundant(>l%). Based on the criteria on sediment quality evaluation, there is little to moderate loading of organic and inorganic matter.Item Zooplankton composition, abundance and diversity in selected Municipalities of Negros Occidental and AklanAusan, Aisa Marie U. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2015-06)Zooplankton samples were collected last May and June 2014 from 20 stations in the vicinity of Kalibo, Aklan and Pontevedra, San Enrique, and Valladolid, Negros Occidental. Three hundred fifty-three (353) liters of water were filtered per station thru a 5m vertical haul of plankton net with a diameter of 30cm and a mesh size of 20μm. Overall zooplankton densities were higher in Negros Occidental, specifically in Valladolid (D=1915.3 cells/L), than in Kalibo, Aklan (D=7.2 cells/L). On the other hand, zooplankton community was most diverse in Kalibo (H’ = 2.6) compared to Valladolid (H’=1.3), San Enrique (H’=2.1) and Pontevedra (H’=1.8). Jaccard’s similarity index was highest between Pontevedra and San Enrique while lowest between Valladolid and Kalibo. In Kalibo there were four major groups of zooplankton. Most dense of these were the tintinnids (3.9 cells/L) followed by copepods (2.5 cells/L), molluscs (7xlOE-lcells/L) and polychaetes (IxlOE-lcell/L). Among the copepods Subeucalanus was the most abundant zooplankton genus. In Pontevedra and San Enrique, Negros Occidental, copepods were the most abundant group with a mean density of 205.3 cells/L and 312.1 cells/L, respectively. The relative abundance ofAcartia (36.5%) and Longipedia (22.6%) made them the dominant zooplankton genera in Pontevedra and San Enrique, respectively. Like in Kalibo, the tintinnids were also the most abundant group in Valladolid (1879.2 cells/L). Copepods followed with a mean density of 35.7 cells/L, while Polychaetes comprised only 4xlOE-l cells/L. Epiplocylis was the most abundant tintinnid in Valladolid while Xystonella in Kalibo. Varying sampling time within months and day is the possible cause of the differences in zooplankton abundance, composition, and diversity since some zooplankton species undergo diel vertical migration.Item Sagip COUGHamilya: Improving prevention, and case management & care seeking in Sitio Durog, Barangay Palaca, MiagaoAsorio, Leslie S.; Clarito, Thea Joy A.; Dolorosa, Rex Victor V.; Marecap, Mitzi N.; Napulan, Andrea Marie N.; Recabar, James Mathew V.; Rodriguez, Sael D.; Velasco, Princess Monic Q. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)According to the 2018 Morbidity Diseases Reports of Miag-ao’s Rural Health Unit (RHU), Acute Respiratory Infection (ARI) is the main cause of morbidity among the children in the municipality. Upon further review, the community that was found to have the greatest number of ARI cases is that of Barangay Palaca. To contribute with the reduction of ARI cases in the said community, Project Sagip COUGHamilya was implemented in Sitio Durog. This project aims to reduce the number of current ARI cases among children aging 2 months to 11 years old by at least 50% after the 6-week project implementation. In order to accomplish such goal, the team utilized strategic components such as: (1) establishment of a core group called FamiLEADERS, (2) honing the ARI case management knowledge and skills of the barangay health workers (BHWs) through FamiLINANG sessions for BHWs, (3) improving the mothers’ & guardians’ knowledge, attitude, and practices (KAP) towards care seeking and case management for ARI through FamiLINANG sessions for mothers, and (4) raising the children’s awareness on cough etiquette, proper handwashing, and other key health practices through LINIStorya sessions. Upon implementation, the project’s outputs include the 80% increase in the ARI case management knowledge and skills of BHWs, 44.47% increase in the KAP of the mothers & guardians towards care seeking and case management for ARI, and 12.5% increase in the children’s awareness towards cough etiquette, proper handwashing, and other key health practices. All these results were statistically found to be significant improvements. In conclusion, the strategies done by the group significantly improved the care seeking, case management, and key health practices for ARI in Sitio Durog, Barangay Palaca. As a result, Projesct Sagip COUGHamilya contributed to the reduction of ARI cases by 80%, far exceeding the goal of the project and deeming it to be very effective and successful.Item Spatial variations of Benthic meiofauna communities from Palompon Bay, Northern LeyteBalasa, Melvy June (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)Meiofauna play a significant role in maintaining ecological balance in marine ecosystems especially in the dynamics of the seagrass meadows. The meiofauna links the upper trophic level with detritus-feeding organisms. Only few studies have been conducted in the Philippines, while no studies have been. This study was conducted in Palompon, northern Leyte in November 2011, to determine the spatial distribution of meiobenthic fauna in the area and to compare community structure in terms of species composition, species density, and species diversity among five stations included in the survey. Twenty taxa were identified with nematodes being numerically dominant, followed by harpacticoid copepods. It also showed the highest mean abundance for the entire habitat type. The overall mean density was 194585.1528 ind. m2, with the highest population at station 4. And the lowest population was recorded at station 2. Soft bottom habitat showed the highest recorded mean density among the 5 stations, Harpacticoids mostly dominated sandy substrate while nematodes were widely distributed.Item Knowledge, attitudes, and practices on dengue vectors of households in Tumagboc Catchment Area, Miagao, IloiloArbizo, Jeric L.; Baldevarona, Rosa Lea S.; Caoyonan, Louise Asuncion D.; Gabasa, Marah Febcy J.; Mapa, Fernie Love D.; Patria, Elaine Joy M.; Tenchavez, Darlene Ruth B.; Trivilegio, Enna Fleur C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)Previous studies have shown that knowledge, attitudes and practices have an association with the dengue vector control. As such, the independence of mosquito population on the sociodemographic characteristics and KAP of the household heads in Tumagboc Catchment Area, Miagao, Iloilo was tested. Based on the results, the household heads generally are unemployed, married females aging 41 to 50. Their households are composed of 4 to 7 members with no or one child below 15 years, and usually receive below P 1999 per month. Most of them are informed about dengue fever, but only one had suffered from it in the last two years. Moreover, they have moderate knowledge, poor attitudes, but good practices regarding dengue prevention. The container index is 2.71 for crude mosquito larvae and pupae population, and 0.39 for Aedes sp. The house index is 9.72, while the Breteaux indexis 13.19. Chi-square analysis at 0.05 level of significance confirmed the independence of mosquito larvae and pupae population from monthly income, dengue information reception, knowledge, attitudes and practices. A solid reason was the untimely conduct of the study in the beginning of the summer season, when stagnant water has dried up and with few vulnerable breeding sites.Item Risk zone mapping of 2010 dengue fever and dengue hemorrhagic fever cases in the Province of Iloilo based on land coverAndrada, Thresdale; Cañonero, Gertrude Mikee; España, Regine Marie; Puno, Abigail; Sarangaya, Dorothy Rose; Tan, Leonard; Tejada, Ma. Corazon (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)Dengue is considered as one of the most important vector-borne diseases in many tropical and subtropical countries. Iloilo Province had 8201 cases in 2010. Heterogeneity in the risk of disease transmission results from spatial heterogeneity in both land use and land cover. Thus, the present study was conducted to identify the risk zones of Dengue Fever and Dengue Hemorrhagic Fever in Province of Iloilo in 2010 based on land cover types by using GIS software. Results of the study show that all land covers in the 6th district pose moderately low risk on dengue incidence. In the case of the 1st district of Iloilo, on the other hand, water bodies have the highest probability of dengue incidence, while the rest of the districts (2nd, 3rd, 4th, 5th). built-up areas have the highest probability of dengue incidence. The land cover type in Iloilo, therefore, with the highest risk for dengue incidence are built up areas due to its high population density, multitude of households and stagnant water areas.Item Gross alpha and gross beta radioactivity and heavy metal concentration profiles of well water of the Calahunan dump site, Mandurriao, Iloilo CityAmarante, Hena Charma; Basquez, Mark Anthony; Brunio, Christian Allan; Dable, Nesel; De la Pena, Pamela-Di; Genterola, Janis; Oredina, Josette Marie; Padayhag, Dave (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2005-03)The study aimed to establish presence of radioactivity and concentration of heavy metals specifically lead and chromium in drinking water from wells within one-kilometer radius from the approximated center of the Calahunan dump site. Results showed that four out of twelve wells have positive results fro gross alpha activity and gross beta activity: wells 2A, 2C, 3A, and 3D. Well 2A, 2C and 3D registered alpha activities of 0.54, 0.033 and 0.369 Bq/L respectively while Well 3A registered beta activity of 1.0 Bq/L. Wells 2A and 3D exceeded the radiological limits set by WHO and PNSDW of 0.1 Bq/L for alpha activity and 1.0 Bq/L for beta activity. These wells that are found adjacent to the perimeter fence of the dump site registered alpha activity (wells 2A, 2C and 3D) and beta activity (well 3A). Radiological activities in all other wells sampled registered less than lower limit of detection. For gamma activity, all wells recorded 137- Cs and 134-Cs activity less than the lower limit of detection. For chromium and lead concentration using FAAS, only well 3C has a chromium concentration of 0.0952 ppm and this exceeded the PNSDW standard for chromium in drinking water (0.05 ppm) FAAS has not recorded any value for lead. For chemical constituent analysis using XRF, two wells in zone 1 (LA and IC), have recorded lead concentration of 0.9 and 0.136 ppm, respectively. In zone two, only well 2D registered a lead concentration of 0.248 ppm. In zone 3, well 3C registered a lead concentration of 0.397 ppm. For chromium concentration, only well 2A in zone 2 has a chromium concentration of 1.79 ppm. The spot interview and ocular inspection found out that the following garbage are disposed of indiscriminately tn the dump site: (1) Television picture tubes, (2) Dysfunctional Computer Monitors, (3) Used syringes and needles, (4) Hospital wastes, (5) Automobile batteries and dry cells, (6) scrap iron, tin cans, paint cans, paint cans, etc., (7) polyethylene bottle, glass bottles, and containers of similar types, and (8) other organic and inorganic wastes. Wells whose values for gross alpha, lead, and chromium concentration exceeded the standard are unsuitable for dinking and other domestic uses. The observation that wells (2A and 3D) near the Calahunan dump site registered higher-than-standard alpha activity may be a good basis to conduct further research particularly on the effect of leachate from dump site.Item Short-term impacts of oil spills on fungal population in a mangrove area in Batad, IloiloAlpas, Donna Bella C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)Oil spills cause extensive damage on the marine environment, ranging from obliteration of its flora and fauna to biomagnifications of toxic components of petroleum. The location of mangroves at the sea-land interface makes the ecosystem susceptible to disturbances such as oil spills. There is a considerable information on the impacts of oil spills on mangrove while impacts on microbial populations, especially fungi, are limited. This study therefore assessed the effect of oil on the diversity and distribution of fungi of oil-contaminated sites in Batad, Iloilo due to Power Barge oil spill last November 8, 2013. The mangrove surface sediments were collected on January 11, 2014 at oil-contaminated sites in Batad, Iloilo and uncontaminated site in Ajuy, Iloilo. Sample were processed based on Pepper and Gerba (2004). Colony forming units per gram of soil (CFU/g) was used to express yeasts and filamentous fungi. On PDA medium, more fungal isolates were isolated at oil-contaminated site compared with uncontaminated site at 14 and 12 species respectively, there was also a higher Shannon Index of Diversity at 2.4858 and 2.4582, respectively. Among the filamentous fungi, three genera were represented by Aspergillus. Penicillium, and Cladosporium. However, Aspergillus was more diverse with 10 species. Six species were found only at the oil-contaminated sites - Aspergillus sp 2. Aspergillus sp 1, Cladosporium cladosporioides, Penicillium sp 2, Penicillium sp 3 and Yeast sp 2 while four species are found in the unoiled site - Penicillium cf. lapidosum, Aspergillus sp 3, Aspergillus sp 4 Penicillium sp 1. Furthermore, there is a low index of similarity as shown by Jaccards coefficient of similarity (J=0.44) and Sorensen coefficient of similarity ( C=0.61). Fungal load was higher at oil-contaminated soils compared with uncontaminated soils at 1.3 x 10 - CFU/g and 6.0 x IO3 CFU/g, respectively. Bushnell Haas Agar yielded more fungi isolates isolated from oil-contaminated sites compared with uncontaminated site of 4 and 3 species respectively. Furthermore, a higher Shannon Index Diversity (H) was observed at oil-contaminated sites (1.3298) than those at uncontaminated site (0.6364) suggesting that there are more species of fungi capable to degrade the hydrocarbons and utilize it as a source of energy. The overall frequency of distribution of the major fungal genera in both oil-contaminated and uncontaminated sites showed that only Dueteromycota was isolated and dominated in both sites and in both media, the PDA and Bushnell Haas Agar. The major genera isolated in PDA medium included Aspergillus, Penicillium, Cladosporium, and yeast. In Bushnell Haas Agar, Aspergillus and yeast were the major genera isolated and are seen in both sites. The frequency of occurrence of various species in uncontaminated and oil-contaminated sites were not significantly different. The Deuteromycota were the dominant group that were isolated on all sites using two media. The overall result indicate the stimulating effect of oil are observed in the higher fungal load in oil- contaminated site.Item Salinity tolerance of the angelwing clam, Pholas orientalis larvaeAguaras, Lovie Grace P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)Pholas orientalis, locally known as “diwal” is one of the commercially important bivalves in the Philippines due to its delicate and sweet taste. An experiment was conducted to determine the salinity tolerance of the 24-hour-old larvae of this clam when reared in different salinity levels of 25, 30, 35, and 40 ppt. The experiment lasted for 15 days at a rearing temperature of 25° C - 27° C. Determination of survival rates and water change were done twice every week. The data gathered were then analyzed using one-way ANOVA. Results showed that survival rates were significantly high at salinities of 30-35 ppt and were significantly low at salinity levels of 25 and 40 ppt. It was also observed that there was an increasing trend of mortality with prolonged exposure to different salinity levels. This could be attributed to the limited osmoregulatory capabilities of the larvae. When reared at low salinity levels, larvae were faced with the danger of too much water entry which eventually led to death. Meanwhile, when reared at high salinity levels, the larvae died due to outward movement of water which resulted to dehydration. Information on the salinity levels that could be tolerated by diwal larvae will be an important factor in the development of larval rearing techniques for successful hatchery operations. The juveniles or seeds produced in the hatchery could be used to restock the depleted resources in the wild.Item Prevalence of Ascaris lumbricoides, Trichuris trichiura, and Enterobius vermicularis in the hands of preschool children in day care centers in Miagao, IloiloAbellon, Rupert L., III (Division of Biological Sciences, Bachelor of Science in Public Health, University of the Philippines Visayas, 2002-03)The hands of 199 preschool children in Day Care Centers of Miag-ao, Iloilo were swabbed and examined for the presence of parasitic ova, specifically soil-transmitted parasites. Prevalence rates of parasitic ova were determined and tested for significant differences with variables that include age, sex, parent’s educational level, and location of the day care center of the preschoolers. Ascaris had a prevalence rate of 24.12% (48), Enterobius with 1.51% (3) and Trichuris with 1.01% (2). Toxocara canis had a 6.03% prevalence (12) in the hands of preschoolers. Using the Chi-square test to test for differences, it was shown that there were no significant differences in the presence of parasitic ova in the hands among preschoolers with regards to age, sex, and parent’s educational level except for location of the day care center. There was a significant difference in the prevalence rate of parasitic ova in the hands of preschoolers in coastal areas and those in non-coastal areas. Preschoolers in the coastal areas had a higher prevalence rate (29.82%) compared to those in the non-coastal areas (16.47%). With a sample of 121 stools from the same group of preschoolers, the study showed that there was no direct association between the presence of Ascaris ova in the hands and their presence in stools.