Undergraduate Special Problem
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Item Spatial variations of Benthic meiofauna communities from Palompon Bay, Northern LeyteBalasa, Melvy June (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)Meiofauna play a significant role in maintaining ecological balance in marine ecosystems especially in the dynamics of the seagrass meadows. The meiofauna links the upper trophic level with detritus-feeding organisms. Only few studies have been conducted in the Philippines, while no studies have been. This study was conducted in Palompon, northern Leyte in November 2011, to determine the spatial distribution of meiobenthic fauna in the area and to compare community structure in terms of species composition, species density, and species diversity among five stations included in the survey. Twenty taxa were identified with nematodes being numerically dominant, followed by harpacticoid copepods. It also showed the highest mean abundance for the entire habitat type. The overall mean density was 194585.1528 ind. m2, with the highest population at station 4. And the lowest population was recorded at station 2. Soft bottom habitat showed the highest recorded mean density among the 5 stations, Harpacticoids mostly dominated sandy substrate while nematodes were widely distributed.Item Knowledge, attitudes, and practices on dengue vectors of households in Tumagboc Catchment Area, Miagao, IloiloArbizo, Jeric L.; Baldevarona, Rosa Lea S.; Caoyonan, Louise Asuncion D.; Gabasa, Marah Febcy J.; Mapa, Fernie Love D.; Patria, Elaine Joy M.; Tenchavez, Darlene Ruth B.; Trivilegio, Enna Fleur C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)Previous studies have shown that knowledge, attitudes and practices have an association with the dengue vector control. As such, the independence of mosquito population on the sociodemographic characteristics and KAP of the household heads in Tumagboc Catchment Area, Miagao, Iloilo was tested. Based on the results, the household heads generally are unemployed, married females aging 41 to 50. Their households are composed of 4 to 7 members with no or one child below 15 years, and usually receive below P 1999 per month. Most of them are informed about dengue fever, but only one had suffered from it in the last two years. Moreover, they have moderate knowledge, poor attitudes, but good practices regarding dengue prevention. The container index is 2.71 for crude mosquito larvae and pupae population, and 0.39 for Aedes sp. The house index is 9.72, while the Breteaux indexis 13.19. Chi-square analysis at 0.05 level of significance confirmed the independence of mosquito larvae and pupae population from monthly income, dengue information reception, knowledge, attitudes and practices. A solid reason was the untimely conduct of the study in the beginning of the summer season, when stagnant water has dried up and with few vulnerable breeding sites.Item Risk zone mapping of 2010 dengue fever and dengue hemorrhagic fever cases in the Province of Iloilo based on land coverAndrada, Thresdale; Cañonero, Gertrude Mikee; España, Regine Marie; Puno, Abigail; Sarangaya, Dorothy Rose; Tan, Leonard; Tejada, Ma. Corazon (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)Dengue is considered as one of the most important vector-borne diseases in many tropical and subtropical countries. Iloilo Province had 8201 cases in 2010. Heterogeneity in the risk of disease transmission results from spatial heterogeneity in both land use and land cover. Thus, the present study was conducted to identify the risk zones of Dengue Fever and Dengue Hemorrhagic Fever in Province of Iloilo in 2010 based on land cover types by using GIS software. Results of the study show that all land covers in the 6th district pose moderately low risk on dengue incidence. In the case of the 1st district of Iloilo, on the other hand, water bodies have the highest probability of dengue incidence, while the rest of the districts (2nd, 3rd, 4th, 5th). built-up areas have the highest probability of dengue incidence. The land cover type in Iloilo, therefore, with the highest risk for dengue incidence are built up areas due to its high population density, multitude of households and stagnant water areas.Item Gross alpha and gross beta radioactivity and heavy metal concentration profiles of well water of the Calahunan dump site, Mandurriao, Iloilo CityAmarante, Hena Charma; Basquez, Mark Anthony; Brunio, Christian Allan; Dable, Nesel; De la Pena, Pamela-Di; Genterola, Janis; Oredina, Josette Marie; Padayhag, Dave (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2005-03)The study aimed to establish presence of radioactivity and concentration of heavy metals specifically lead and chromium in drinking water from wells within one-kilometer radius from the approximated center of the Calahunan dump site. Results showed that four out of twelve wells have positive results fro gross alpha activity and gross beta activity: wells 2A, 2C, 3A, and 3D. Well 2A, 2C and 3D registered alpha activities of 0.54, 0.033 and 0.369 Bq/L respectively while Well 3A registered beta activity of 1.0 Bq/L. Wells 2A and 3D exceeded the radiological limits set by WHO and PNSDW of 0.1 Bq/L for alpha activity and 1.0 Bq/L for beta activity. These wells that are found adjacent to the perimeter fence of the dump site registered alpha activity (wells 2A, 2C and 3D) and beta activity (well 3A). Radiological activities in all other wells sampled registered less than lower limit of detection. For gamma activity, all wells recorded 137- Cs and 134-Cs activity less than the lower limit of detection. For chromium and lead concentration using FAAS, only well 3C has a chromium concentration of 0.0952 ppm and this exceeded the PNSDW standard for chromium in drinking water (0.05 ppm) FAAS has not recorded any value for lead. For chemical constituent analysis using XRF, two wells in zone 1 (LA and IC), have recorded lead concentration of 0.9 and 0.136 ppm, respectively. In zone two, only well 2D registered a lead concentration of 0.248 ppm. In zone 3, well 3C registered a lead concentration of 0.397 ppm. For chromium concentration, only well 2A in zone 2 has a chromium concentration of 1.79 ppm. The spot interview and ocular inspection found out that the following garbage are disposed of indiscriminately tn the dump site: (1) Television picture tubes, (2) Dysfunctional Computer Monitors, (3) Used syringes and needles, (4) Hospital wastes, (5) Automobile batteries and dry cells, (6) scrap iron, tin cans, paint cans, paint cans, etc., (7) polyethylene bottle, glass bottles, and containers of similar types, and (8) other organic and inorganic wastes. Wells whose values for gross alpha, lead, and chromium concentration exceeded the standard are unsuitable for dinking and other domestic uses. The observation that wells (2A and 3D) near the Calahunan dump site registered higher-than-standard alpha activity may be a good basis to conduct further research particularly on the effect of leachate from dump site.Item Short-term impacts of oil spills on fungal population in a mangrove area in Batad, IloiloAlpas, Donna Bella C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)Oil spills cause extensive damage on the marine environment, ranging from obliteration of its flora and fauna to biomagnifications of toxic components of petroleum. The location of mangroves at the sea-land interface makes the ecosystem susceptible to disturbances such as oil spills. There is a considerable information on the impacts of oil spills on mangrove while impacts on microbial populations, especially fungi, are limited. This study therefore assessed the effect of oil on the diversity and distribution of fungi of oil-contaminated sites in Batad, Iloilo due to Power Barge oil spill last November 8, 2013. The mangrove surface sediments were collected on January 11, 2014 at oil-contaminated sites in Batad, Iloilo and uncontaminated site in Ajuy, Iloilo. Sample were processed based on Pepper and Gerba (2004). Colony forming units per gram of soil (CFU/g) was used to express yeasts and filamentous fungi. On PDA medium, more fungal isolates were isolated at oil-contaminated site compared with uncontaminated site at 14 and 12 species respectively, there was also a higher Shannon Index of Diversity at 2.4858 and 2.4582, respectively. Among the filamentous fungi, three genera were represented by Aspergillus. Penicillium, and Cladosporium. However, Aspergillus was more diverse with 10 species. Six species were found only at the oil-contaminated sites - Aspergillus sp 2. Aspergillus sp 1, Cladosporium cladosporioides, Penicillium sp 2, Penicillium sp 3 and Yeast sp 2 while four species are found in the unoiled site - Penicillium cf. lapidosum, Aspergillus sp 3, Aspergillus sp 4 Penicillium sp 1. Furthermore, there is a low index of similarity as shown by Jaccards coefficient of similarity (J=0.44) and Sorensen coefficient of similarity ( C=0.61). Fungal load was higher at oil-contaminated soils compared with uncontaminated soils at 1.3 x 10 - CFU/g and 6.0 x IO3 CFU/g, respectively. Bushnell Haas Agar yielded more fungi isolates isolated from oil-contaminated sites compared with uncontaminated site of 4 and 3 species respectively. Furthermore, a higher Shannon Index Diversity (H) was observed at oil-contaminated sites (1.3298) than those at uncontaminated site (0.6364) suggesting that there are more species of fungi capable to degrade the hydrocarbons and utilize it as a source of energy. The overall frequency of distribution of the major fungal genera in both oil-contaminated and uncontaminated sites showed that only Dueteromycota was isolated and dominated in both sites and in both media, the PDA and Bushnell Haas Agar. The major genera isolated in PDA medium included Aspergillus, Penicillium, Cladosporium, and yeast. In Bushnell Haas Agar, Aspergillus and yeast were the major genera isolated and are seen in both sites. The frequency of occurrence of various species in uncontaminated and oil-contaminated sites were not significantly different. The Deuteromycota were the dominant group that were isolated on all sites using two media. The overall result indicate the stimulating effect of oil are observed in the higher fungal load in oil- contaminated site.Item Salinity tolerance of the angelwing clam, Pholas orientalis larvaeAguaras, Lovie Grace P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)Pholas orientalis, locally known as “diwal” is one of the commercially important bivalves in the Philippines due to its delicate and sweet taste. An experiment was conducted to determine the salinity tolerance of the 24-hour-old larvae of this clam when reared in different salinity levels of 25, 30, 35, and 40 ppt. The experiment lasted for 15 days at a rearing temperature of 25° C - 27° C. Determination of survival rates and water change were done twice every week. The data gathered were then analyzed using one-way ANOVA. Results showed that survival rates were significantly high at salinities of 30-35 ppt and were significantly low at salinity levels of 25 and 40 ppt. It was also observed that there was an increasing trend of mortality with prolonged exposure to different salinity levels. This could be attributed to the limited osmoregulatory capabilities of the larvae. When reared at low salinity levels, larvae were faced with the danger of too much water entry which eventually led to death. Meanwhile, when reared at high salinity levels, the larvae died due to outward movement of water which resulted to dehydration. Information on the salinity levels that could be tolerated by diwal larvae will be an important factor in the development of larval rearing techniques for successful hatchery operations. The juveniles or seeds produced in the hatchery could be used to restock the depleted resources in the wild.Item Prevalence of Ascaris lumbricoides, Trichuris trichiura, and Enterobius vermicularis in the hands of preschool children in day care centers in Miagao, IloiloAbellon, Rupert L., III (Division of Biological Sciences, Bachelor of Science in Public Health, University of the Philippines Visayas, 2002-03)The hands of 199 preschool children in Day Care Centers of Miag-ao, Iloilo were swabbed and examined for the presence of parasitic ova, specifically soil-transmitted parasites. Prevalence rates of parasitic ova were determined and tested for significant differences with variables that include age, sex, parent’s educational level, and location of the day care center of the preschoolers. Ascaris had a prevalence rate of 24.12% (48), Enterobius with 1.51% (3) and Trichuris with 1.01% (2). Toxocara canis had a 6.03% prevalence (12) in the hands of preschoolers. Using the Chi-square test to test for differences, it was shown that there were no significant differences in the presence of parasitic ova in the hands among preschoolers with regards to age, sex, and parent’s educational level except for location of the day care center. There was a significant difference in the prevalence rate of parasitic ova in the hands of preschoolers in coastal areas and those in non-coastal areas. Preschoolers in the coastal areas had a higher prevalence rate (29.82%) compared to those in the non-coastal areas (16.47%). With a sample of 121 stools from the same group of preschoolers, the study showed that there was no direct association between the presence of Ascaris ova in the hands and their presence in stools.Item Physico-chemical analysis and assessment of river waters discharged by sugar milling companies in Negros OccidentalAlger, Niña Xiamima A.; Arinzol, Shaira S.; Bobon, Steve Paolo P.; Daquilanea, Mikkaela C.; de Leon, Sarah C.; Hilado, Paolo C.; Lastica, Ariel C.; Manderico, Regine Viel D. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-03)This cross-sectional study was focused on the river waters discharged by six sugar milling companies in Negros Occidental. These companies utilize river water in order to operate and may alter the characteristics of the waters. This study aims to analyze and assess the physical characteristics, namely pH, oil and grease, temperature, salinity, turbidity, and color, and the chemical characteristics, namely biological oxygen demand (BOD), dissolved oxygen (DO), ammonia, phosphorus, and lead content, of these waters in order to identify the alterations upon its usage, detect abnormal characteristics, and eventually determine possible environmental and health hazards. Three samplings were done for each river’s downstream and upstream portions. The following tests and instruments were performed and were used on the samples: pH Meter for pH, DO Meter for DO, Wrinkler’s Test for BOD, PE Ascorbic Acid Test for phosphorus, Spectrophotometry for turbidity, Phenate Test for Ammonia, Hand Refractometer for salinity, Visual Comparison for color, Digital Thermometer for temperature, Flame Atomic Absorption Spectrophotometry for lead content, and Petroleum Ether Extraction Method for oil and grease. It was discovered most of the discharged river waters apparently had decreased pH and DO and increased BOD, temperature, salinity, ammonia, phosphorus, turbidity, and oil and grease content. Lead was detected on the all of the downstream portions. The pH and DO of most of the discharged river waters were ostensibly below the acceptable values while the oil and grease, ammonia, phosphorus, salinity, and lead content were above the acceptable ones. Temperature, color, and turbidity were the only parameters which fell within the acceptable values. Therefore, there can seemingly be environmental and health hazards posed on the residents living downstream since they are having direct or indirect contact with the contaminated or polluted water. It is recommended that laws should be made with regards to how the residents use the water and that the implementation of existing laws should be strengthened. Lastly, it is strongly advised that the DENR should increase the number of parameters analyzed during their regular monitoring, that the SMCs should improve the treatment of the waters, and that the effects of farmlands and communities on the river should be identified.Item Microbiological water analysis of selected point sources in Iloilo CityAlama, Jannie Mae G.; Calitis, Ma. Socorro Fatima P.; Cervantes, Ria Angela E.; Garcia, Marian Aisa C.; Otico, Pelita A.; Pacheco, Donna Ginn G.; Taquiso, Jezreel l. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2006-04)The recreational use of the Iloilo River according to the Iloilo River Master Plan entails various studies to support it being safe for swimming and other activities. The Department of Environment and Natural Resources (DENR) Region VI has done very few studies to determine this particular aspect of the Iloilo River. Thus, the present study was conducted to determine the bacteriologic content of water in selected point sources in Iloilo River, Iloilo City and the possible correlations to the incidence of health risks. Specifically the study aims to: (1) determine significant differences in the physico-chemical characteristics of water samples from each point source; (2) to determine the total and fecal coliform counts number for each water sample from the selected point sources; (2) detect the presence or absence of E. coli, Salmonella spp., and culturable Vibrios in water in selected point sources; (3) describe the incidence of health risks; and (4) correlate the heterotrophic plate count for total coliforms, Most Probable Number for Fecal Coliforms and the number of symptomatic respondents. The results of the study showed that the total coliform count for Point source A (HPC=6000/l00 ml) is higher compared to the standard set by the DENR (5000/100 ml) while Point source B and C are within the range. The concentrations of E. coli, in water samples A, B, and C (2400 MPN/100 ml sample) were found to be higher than the DENR standard for fecal coliforms for Class C Waters - fresh water, (200 MPN/lOOml sample). Although results indicate that the river water has high fecal contamination, there were no significant correlations between heterotrophic plate counts, Most Probable Number for Fecal Coliforms and the number of symptomatic respondents for each point source.Item Intestinal parasitic infections in children living in selected barangays with urban slums along Iloilo RiverAguro, Eric D.; Banaag, Arlene D.; Jacobe, Venus N.; Ordaniel, Lilac Mared A.; Taleon, Kathleen Joy B.; Yandra, Marnelle Gay A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2006-03)This cross-sectional study was performed to determine the intestinal parasitic infection in children age 0-13 years old in three selected barangays with urban slums located alongside Iloilo River. A total of 127 stool samples from 65 males and 62 females were examined by utilizing formalin-ether concentration technique (FECT) and direct wet mount thereafter using Lugol's solution. It was found out that 59.8% of the children examined were positive for one or more intestinal parasites. The most frequent soil-transmitted helminth observed was Ascaris lumbricoides (48.0%) followed by Trichuris trichiura (34.6%). Pathogenic protozoans determined were Entamoeba histolytica (9.4%) and Giardia lamblia (0.8%). Other nonpathogenic species identified were Entamoeba coli (15.7%), Blastocystis hominis (3.1%) and lodamoeba butschlii (0.8%). Parasitic infection with single species had the highest frequency of 31.5% compared to multiple infection (28.3%). The relatively high prevalence of these infections among children living three selected barangays with urban slums alongside Iloilo River was found to be independent of factors the respondents’ sex (p=0.106), age group (p=1.691), and consistency of stool sample (p=1.835). Moreover, the degree of infection in terms of sex (p=3.174), age (p=9.136), and consistency of stool samples (p=2.798) were not statistically different from each other. The high prevalence rate of pathogenic intestinal parasitic infection may have also been influenced by factors other than the ones considered, such as environmental and socio-demographic factors. The study highly suggests mass treatment and preventive measures such as health education of the community