Undergraduate Special Problem

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Abundance, composition and distribution of phytoplankton in West Central Sulu Sea
Bria, Jed B. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-11)
A total of 28 stations were sampled for abundance, composition and distribution of phytoplankton in the West Central Sulu Sea. The samples were taken from 15-30 April 2007. Samples were collected by filtering 50L of surface water through a 20 pm mesh plankton net. A total of 96 phytoplankton genera were identified and were grouped into four major taxa. The overall mean density was 444.6 cells/L. Diatoms were the most abundant, with the mean density of 213.5 cells/L. The most abundant diatom genus was Chaetoceros. Blue-green algae showed the second-highest mean density of 127.6 cells/L. Trichodesmium was the most abundant genus among the two blue-green algae identified. Trichodesmium was also the most abundant genus, comprising 28.1% of all phytoplankton identified. Dinoflagellates and silicoflagellates were ranked third and fourth among the four major taxa with 77.5 cells/L and 28 cells/L, respectively. Protoperidinium was the most abundant dinoflagellate, while Amphorella was the most abundant silicoflagellate. They made up 6.2% and 1.2 % of all phytoplankton identified, respectively. The stations located along the coast of Palawan and near the Tubattaha Reef Marine National Park showed the highest phytoplankton concentrations.
Bacteriological survey and detection of Escherichia coli, Salmonella spp., Streptococcus spp., and Vibrio spp. in bottom sediments from two sites in Iloilo River
Barrera, Christopher (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-04)
Bacterial contamination in the Iloilo river occurs because of various human activities. The assessment of the river was done using microbiological parameters (HPC, TC, FC, and FS) for 7 months in Sooc Bridge and Carpenter’s Bridge using bottom sediments for samples. Sediment was used because bacteria accumulate over time in the sediment due to high amounts of organic and inorganic matter in the substrate and this leads to better survivability of bacteria. The values obtained from microbiological parameters investigated are generally higher in Sooc Bridge. HPC results show that the higher mean value was in Sooc Bridge(7.43 x 108 CFU/lOOg). The levels of TC on both sampling sites ranged from 9.5 x 102to 2.40 x 104 CFU/100g. For TC, Sooc has the higher mean (1.76 x 104 CFU/lOOg) for 7 months. FC values ranged from 3.0 x 101 to 2.4 x 104 CFU/lOOg. Sooc bridge has the higher mean(1.17 x 104 CFU/lOOg) between the 2 sites. Lastly, the FS value range from 2.9 x 103 to 2.4 x 104 CFU/lOOg. Also, Sooc Bridge has the higher mean value (1.58 x 104 CFU/lOOg). There are no significant differences on the microbiological parameters used in each site. The FC/FS ratio revealed that mixed fecal materials contaminate the river. Among the four pathogenic bacteria detected, Salmonella spp. was the most abundant for 7 months (63-65%) and E. coli was the least abundant(>l%). Based on the criteria on sediment quality evaluation, there is little to moderate loading of organic and inorganic matter.
Floristic composition and diversity of mangroves in selected sites in the province of Antique, Panay Island, Philippines.
Balinas, Lucelle V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2006-03)
Mangroves in the Philippines are in constant danger due to innumerable disturbances in the environment. The study endeavored to assess the floristic composition and diversity of the mangroves in the province of Antique through transect and ocular survey in the randomly selected sites throughout the province. Diversity in the region is high with the presence of 3 1 true mangrove species represented by 16 families and 14 genera. The species include Acanthus ebracteatus, A. ilicifolius, and A. volubilis from Family Acanthaceae, Avicennia alba, A. marina. A. officinalis, and A. rhumphiana from Family Avicenniaceae, Camptostemon philippinensis from Family Bombaceae, Lumnitzera littorea and Lumnitzera racemosa from Family Combretaceae, Excoecaria agallocha from Family Euphorbiaceae, Pemphis acidula from Family Lythraceae, Xylocarpus granatum and Xylocarpus moluccensis from Family Meliaceae, Aegiceras corniculatum and Aegiceras floridum from Family Myrsinaceae, Osbornia octodonta of Family Myrtaceae, Nypa fruticans of Family Palmae, Bruguieria cvlindrica. B. gymnorrhiza, and B. sexangula, Ceriops decandra, C. tagal, Rhizophora apiculata. R. mucronata and R. stylosa from Family Rhizophoraceae, Scyphiphora hydrophvllaceae from Family Rubiaceae, Sonneratia alba, S. caseolaris and S. ovata from Family Sonneratiaceae, and Heritiera littoralis from Family Sterculiaceae . The Shannon Index of Diversity used to determine the measure of diversity in the area is correlated with Pielou's Equitability Index for Evenness. The measures of indices showed that diversity and evenness of the mangal area in the province are directly related, with values of 0.73232 and 0.51163, respectively. Caluya showed the highest diversity index of 1.0132, which consisted of 28 species from 13 families and 15 genera, while Bugasong has a relatively low diversity of 0.0486, denoted by 6 species from 4 families and 4 genera. In general, the high variations in the geomorphology of coastal areas in Antique provided a wide range of substrata for colonization of mangroves that contributed to the high species diversity.
Zooplankton composition, abundance and diversity in selected Municipalities of Negros Occidental and Aklan
Ausan, Aisa Marie U. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2015-06)
Zooplankton samples were collected last May and June 2014 from 20 stations in the vicinity of Kalibo, Aklan and Pontevedra, San Enrique, and Valladolid, Negros Occidental. Three hundred fifty-three (353) liters of water were filtered per station thru a 5m vertical haul of plankton net with a diameter of 30cm and a mesh size of 20μm. Overall zooplankton densities were higher in Negros Occidental, specifically in Valladolid (D=1915.3 cells/L), than in Kalibo, Aklan (D=7.2 cells/L). On the other hand, zooplankton community was most diverse in Kalibo (H’ = 2.6) compared to Valladolid (H’=1.3), San Enrique (H’=2.1) and Pontevedra (H’=1.8). Jaccard’s similarity index was highest between Pontevedra and San Enrique while lowest between Valladolid and Kalibo. In Kalibo there were four major groups of zooplankton. Most dense of these were the tintinnids (3.9 cells/L) followed by copepods (2.5 cells/L), molluscs (7xlOE-lcells/L) and polychaetes (IxlOE-lcell/L). Among the copepods Subeucalanus was the most abundant zooplankton genus. In Pontevedra and San Enrique, Negros Occidental, copepods were the most abundant group with a mean density of 205.3 cells/L and 312.1 cells/L, respectively. The relative abundance ofAcartia (36.5%) and Longipedia (22.6%) made them the dominant zooplankton genera in Pontevedra and San Enrique, respectively. Like in Kalibo, the tintinnids were also the most abundant group in Valladolid (1879.2 cells/L). Copepods followed with a mean density of 35.7 cells/L, while Polychaetes comprised only 4xlOE-l cells/L. Epiplocylis was the most abundant tintinnid in Valladolid while Xystonella in Kalibo. Varying sampling time within months and day is the possible cause of the differences in zooplankton abundance, composition, and diversity since some zooplankton species undergo diel vertical migration.
Effects of Chromolaena odorata leaves as amendments to poultry manure and inorganic fertilizer to improve growth, yield, biomass accumulation and inorganic content of tomato (Lycopersicum esculentum)
Balinas, Chona Mae D. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2012-04)
Chromolaena odorata (a weed in the family Asteraceae) has been shown to enhance the buildup of organic matter in a fallow system in tropical soil. Therefore, the aim of this study was to to determine the effects of Chromolaena odorata leaves as an amendment to poultry manure and triple 14 inorganic fertilizer on the growth, yield, biomass accumulation and inorganic contents of Lycopersicum esculentum. The field experiment was conducted at Agsanayan Buenavista Guimaras where the weed has wide distribution. The leaves of C. odorata were air dried and composted using Berkeley method. The compost obtained was used as an amendment to either poultry manure and inorganic fertilizer and was tested on tomato for three months in RCBD. Obtained results proved that tested treatments with amendments of C. odorata were significantly higher in lengths of shoots and roots, flower and fruit yield, amount of biomass accumulated and inorganic contents of tomato than those with no amendments of C. odorata. Therefore, C. odorata is an efficient amendment to both organic and inorganic fertilizers. It was suggested that the weed should be utilized to serve as a green manure.
Spatial variations of Benthic meiofauna communities from Palompon Bay, Northern Leyte
Balasa, Melvy June (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)
Meiofauna play a significant role in maintaining ecological balance in marine ecosystems especially in the dynamics of the seagrass meadows. The meiofauna links the upper trophic level with detritus-feeding organisms. Only few studies have been conducted in the Philippines, while no studies have been. This study was conducted in Palompon, northern Leyte in November 2011, to determine the spatial distribution of meiobenthic fauna in the area and to compare community structure in terms of species composition, species density, and species diversity among five stations included in the survey. Twenty taxa were identified with nematodes being numerically dominant, followed by harpacticoid copepods. It also showed the highest mean abundance for the entire habitat type. The overall mean density was 194585.1528 ind. m2, with the highest population at station 4. And the lowest population was recorded at station 2. Soft bottom habitat showed the highest recorded mean density among the 5 stations, Harpacticoids mostly dominated sandy substrate while nematodes were widely distributed.
Fungal pathogen associated with Rhizophora stylosa: A comparative study between oiled and unoiled sites within Taklong Island National Marine Reserve (TINMAR), Nueva Valencia, Guimaras
Balanga-an, Kristoffer (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)
Mangroves, refer to an assemblage of trees and shrubs that inhabit the coastal intertidal zone in tropical and subtropical regions. They help in the soil formation, protection, and stabilization of our coastal environment as well as serve as shelter and breeding grounds for some of our marine organisms. A mangrove habitat represents the borderline between land and sea and is considered as one of the principal places where spilled oil and associated impacts converge. Oil spill threaten mangroves by predisposing the plants to fungal attacks thereby reducing their resistance to stress and diseases. Studies also show that certain fungi species are able to utilize oil in contaminated mangrove species. This study generally aims to compare the fungal population found on the diseased leaves of Rhizophora stylosa between oiled and unoiled sites in the Taklong Island National Marine Reserve (TINMAR). It also aims to isolate, characterize and identify fungi present in diseased leaves of Rhizophora stylosa using potato dextrose agar (PDA) culture media and determine their frequency of occurrence. Leaf samples were collected from two sites in TINMAR, one of which was contaminated by oil and the other one was not. Three trees were selected to represent each of the two sampling sites (oiled and unoiled), and to each of the selected trees, three diseased leaves were collected. The results of this study showed the dominance of some species of fungi namely, Aspergillus (40%), Penicillium (20%), Cladosporium (13.33%), Monilia (6.67%), Mycelia (6.67%), Humicola (6.67%), and Pestalotiopsis (6.67%). Five out of these seven genera were isolated from the unoiled site while six of these were isolated from the oiled site. Generally, the fungal population was observed to be greater in the oiled site than in the unoiled. This preliminary data indicate that R. stylosa might still be undergoing stress due to the remaining components of the spilled oil in the area. However, this can only be resolved by further monitoring.
Antimicrobial activity of macrofungal basidiomycetous mycelia against Vibrio spp.
Bagacay, Jann Felnesh Exe V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2023-06)
Aquaculture is vital for global food security, producing 50% of the world’s fish. However, infectious diseases caused by fish pathogens like Vibrio species can lead to significant production losses. To address this issue, antimicrobial agents are commonly used. Macrofungal fruiting bodies and mycelia produce bioactive compounds with antimicrobial properties. This study aims to: (a) assess antimicrobial activity in isolated macrofungal samples against the fish bacterial pathogens Vibrio parahaemolyticus and Vibrio cholerae, (b) compare two methods (agar plug and crude extracts) for antimicrobial activity, (c) determine the minimum inhibitory concentration (MIC) using agar well diffusion and the agar plug methods, and (d) identify the macrofungal isolate with the highest antimicrobial activity. Ten morphospecies were collected from the University of the Philippines Visayas Miagao Campus. The samples were evaluated using the agar plug and agar well diffusion methods. The results showed antimicrobial activity against Vibrio cholerae and Vibrio parahaemolyticus. The activity index ranged from 0 to 0.83 and 0.22 to 0.78 for agar wells, and 0 to 1.00 and 0.70 to 1.30 for agar plugs, against V cholerae and V. parahaemolyticus, respectively. Termitomyces globulus and Trametes sp. 1 exhibited the highest activity indices using the agar plug and agar well diffusion methods, respectively. Statistical analysis confirmed significant differences in antimicrobial activity against both test organisms. These findings demonstrate the potential of macrofungi as a valuable source of antimicrobial compounds for controlling fish pathogens in aquaculture. Further research could lead to the identification and utilization of these compounds to minimize production losses and improve disease management in aquaculture.
Knowledge, attitudes, and practices on dengue vectors of households in Tumagboc Catchment Area, Miagao, Iloilo
Arbizo, Jeric L.; Baldevarona, Rosa Lea S.; Caoyonan, Louise Asuncion D.; Gabasa, Marah Febcy J.; Mapa, Fernie Love D.; Patria, Elaine Joy M.; Tenchavez, Darlene Ruth B.; Trivilegio, Enna Fleur C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)
Previous studies have shown that knowledge, attitudes and practices have an association with the dengue vector control. As such, the independence of mosquito population on the sociodemographic characteristics and KAP of the household heads in Tumagboc Catchment Area, Miagao, Iloilo was tested. Based on the results, the household heads generally are unemployed, married females aging 41 to 50. Their households are composed of 4 to 7 members with no or one child below 15 years, and usually receive below P 1999 per month. Most of them are informed about dengue fever, but only one had suffered from it in the last two years. Moreover, they have moderate knowledge, poor attitudes, but good practices regarding dengue prevention. The container index is 2.71 for crude mosquito larvae and pupae population, and 0.39 for Aedes sp. The house index is 9.72, while the Breteaux indexis 13.19. Chi-square analysis at 0.05 level of significance confirmed the independence of mosquito larvae and pupae population from monthly income, dengue information reception, knowledge, attitudes and practices. A solid reason was the untimely conduct of the study in the beginning of the summer season, when stagnant water has dried up and with few vulnerable breeding sites.
Community structure of mangroves in selected barangays in Sebaste, Antique
Alorro, Mary Juandin Grace F. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2007-03)
Mangroves are higher plants that are primarily found predominantly in the intertidal areas such as estuaries and lagoons of the tropical and subtropical shorelines. This study was conducted to assess the community structure of mangroves in Barangay Callan, Barangay Idio, Barangay Poblacion, Barangay Abiera, and Barangay Bacalan, Sebaste, Antique, using the transect line method. Specifically, it aimed to determine selected physical and chemical factors such as salinity, pH, temperature for water and soil as well as characterization of the substrate; identify and classify true mangrove species present in the area; and describe the community structure in terms of stand basal area, stems per hectare, relative density, relative frequency, relative dominance, importance value, Shannon index of diversity, zonation patterns, seedling and sapling density. No study has been done to document the community structure of mangroves in Sebaste, Antique thus, this study was done to provide vital information needed for its proper management. Three stations were selected in Sebaste, Antique. Four (4) species of true mangroves were identified, belonging to three (3) families: Palmae - Nypa fruticans; Rhizophoraceae - Rhizophora apiculata, Bruguiera gymnorrhiza; and Euphorbiaceae - Excoecaria agallocha. The study area had a water salinity range of 0.6 ppt to 15.3 ppt; water temperature range of 28.3°C to 35.4°C; water pH range of 7.1 to 7.7; soil temperature range of 28.3°C to 34.7°C; and soil pH range of 6.5 to 7.2. Basal area ranges from 3.16 m2/ha (Rhizophora apiculata) to 5.88 m2/ha (Excoecaria agallochd) to 8.19 m2/ha (Bruguiera gymnorrhiza) . The species with the highest average of stems per hectare were Excoecaria agallocha and Bruguiera gymnorrhiza with 300 as well as the highest average density value of 100 for Excoecaria agallocha. Nypa fruticans had the highest relative frequency and relative dominance. Among the four species, the most important species was Nypa fruticans. The Shannon Index of Dominance was 0.403 which was quite low in terms of diversity as influenced by the dominance of Nypa fruticans.

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