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Undergraduate Special Problem

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/30

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Committee Chair: search.filters.chair.Padilla, Philip Ian P.SDG: search.filters.sdg.SDG 14 - Life below water

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    Endophytic fungi from Halimeda macroloba and Sargassum sp.in Nueva Valencia Nueva Valencia, Guimaras, Philippines
    Alemania, Darlene Kris V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)
    Endophytic fungi of marine algae in the Philippines have not been widely explored despite the fact that marine fungi associated with algae are the second most diverse. This study aimed to conduct a preliminary investigation on the occurrence of endophytic fungi from Halimeda macroloba and Sargassum sp. In this study fungal endophytes were isolated from Halimeda macroloba and Sargassum sp. collected from Nueva Valencia, Guimaras. Broad-leaf macroalagal samples were surface sterilized and cultured on PDA seawater media at 37°C. Isolated fungal endophytes were identified through colony and microscopic characteristics. Twenty one distinct endophytic fungi were isolated, 17 of which were identified up to the genus level with the most endophytes belonging to the genus Aspergillus. Fungal endophytes obtained were mostly ascomycetes specifically Aspergillus sp., one hyphomycete and three unidentified fungi with sterile mycelia were present. The brown algae, Sargassum sp., had a higher diversity compared to the green algae Halimeda macroloba in spite of having a lower isolation frequency. In terms of similarity, the two algal hosts had a low index of similarity with J= 10%. This study contributes to the growing data regarding diversity and abundance of fungal endophyte species from marine macroalgae in the Philippines. This is the first study conducted on fungal endophyte assemblages of algae species in Western Visayas.
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    Effects of crude methanolic extract of purple nutgrass (Cyperus rotundus L.) on the maturation of golfish oocytes
    Acebuque, Florel May C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)
    Cyperus rotundus L. is a pernicious weed, which is traditionally used for different medical purposes and is locally recommended for antifertility purposes. A number of pharmacologically important constituents have been previously isolated from the plant. This study aims to test the effects of the crude methanolic extract of C. rotundus L. on the maturation of goldfish oocytes. The extract is found to induce 100% maturation at 5 mg/mL concentration. However, it becomes toxic at higher concentration. All goldfish oocytes (100% ) has died at 20 mg/mL concentration. The steroids and flavonoids present in the plant may be responsible for inducing goldfish oocyte maturation, whereas the alkaloids may be toxic to the oocytes. Further studies using mammalian systems in vivo and characterization of individual constituents are recommended.