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    Antimicrobial activity of macrofungal basidiomycetous mycelia against Vibrio spp.
    Bagacay, Jann Felnesh Exe V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2023-06)
    Aquaculture is vital for global food security, producing 50% of the world’s fish. However, infectious diseases caused by fish pathogens like Vibrio species can lead to significant production losses. To address this issue, antimicrobial agents are commonly used. Macrofungal fruiting bodies and mycelia produce bioactive compounds with antimicrobial properties. This study aims to: (a) assess antimicrobial activity in isolated macrofungal samples against the fish bacterial pathogens Vibrio parahaemolyticus and Vibrio cholerae, (b) compare two methods (agar plug and crude extracts) for antimicrobial activity, (c) determine the minimum inhibitory concentration (MIC) using agar well diffusion and the agar plug methods, and (d) identify the macrofungal isolate with the highest antimicrobial activity. Ten morphospecies were collected from the University of the Philippines Visayas Miagao Campus. The samples were evaluated using the agar plug and agar well diffusion methods. The results showed antimicrobial activity against Vibrio cholerae and Vibrio parahaemolyticus. The activity index ranged from 0 to 0.83 and 0.22 to 0.78 for agar wells, and 0 to 1.00 and 0.70 to 1.30 for agar plugs, against V cholerae and V. parahaemolyticus, respectively. Termitomyces globulus and Trametes sp. 1 exhibited the highest activity indices using the agar plug and agar well diffusion methods, respectively. Statistical analysis confirmed significant differences in antimicrobial activity against both test organisms. These findings demonstrate the potential of macrofungi as a valuable source of antimicrobial compounds for controlling fish pathogens in aquaculture. Further research could lead to the identification and utilization of these compounds to minimize production losses and improve disease management in aquaculture.
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    Nasal colonization and drug-resistance patterns of Staphylococcus aureus and Staphylococcus epidermidis among young residents within the vicinity of Calajunan Dumpsite, Mandurriao, Iloilo City
    Aguila, Julex Rey; Biaoco, Christine; Gayatgay, Christian Faith; Ingalla, Lydimel; Macalalag, Florence Mae; Omar Al-Abdalla, Afnan; Tolentino, Joanna Rose; Torrilla, Marie Stephanie (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)
    Poor disposal and handling of wastes is one of the problems in developing countries as well as in urban places. Leachate, which includes potential pathogens like Staphylococcus aureus and Staphylococcus epidermidis, occurs when solid wastes adsorb moisture and may contaminate ground water sources. The increasing antibiotic resistance of these species poses a great risk to those exposed to the dumpsite. This study generally aimed to investigate nasal colonization by multidrug-resistant S. aureus and S. epidermidis in young residents of Barangay Calajunan, Mandurriao, Iloilo City and to evaluate the relationship between multidrug-resistance of S. aureus and S. epidermidis and possible risk factors such as direct exposure to the dumpsite, close contact with waste-pickers, antibiotic use, hospitalization, skin and soft-tissue infections, and personal hygiene. Nasal swabs were obtained from eighty-three young residents aged 5-14 years. Isolation and identification of Staphylococcus spp. were based on standard conventional biochemical methods such as mannitol fermentation, catalase and coagulase tests. Anti-staphylococcal agents for the detection of drug susceptibility patterns were penicillin G (10 units), oxacillin (1 µg), cefoxitin (30 μg), ampicillin (10 μg), vancomycin (30 μg), erythromycin (15 µg), tetracycline (30 µg), and clindamycin (2 µg). Of the fifty-one isolated presumptively identified staphylocococcal species, 92% (n=49) were identified as S. aureus and 4% (n=2) as S. epidermidis. The prevalence of nasal colonization of S. aureus and S. epidermidis among the study populace was 59% and 2.41%, respectively. Eighty-two percent (n=42) of the S. aureus isolates exhibited susceptibility to tetracycline, while 98% (n=48) exhibited resistance to ampicillin. One hundred percent (n=2) of the S. epidermidis isolates exhibited susceptibility to erythromycin and tetracycline, and resistance to penicillin G and ampicillin. Multidrug-resistant (MDR) strains were phenotypically determined based on non-sensitivity to two or more of the different antimicrobial classes. Sixty-one percent (n=31) of S. aureus isolates and 50% (n=2) of S. epidermidis were MDR strains. Of these staphylococcal isolates, 54.8% (n=17) were resistant to antimicrobials belonging to Penicillins and Glycopeptides with one isolate being S. epidermidis and 16 S. aureus. No significant association between multidrug resistance of S. aureus and S. epidermidis and the risk factors was observed among the study population (p>0.05), however, it was observed that those who bathed and washed more often were more likely to have both nasal colonization and multidrug resistance of S. aureus and S. epidermidis.