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Undergraduate Special Problem

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    Bacteriological survey and detection of Escherichia coli, Salmonella spp., Streptococcus spp., and Vibrio spp. in bottom sediments from two sites in Iloilo River
    Barrera, Christopher (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-04)
    Bacterial contamination in the Iloilo river occurs because of various human activities. The assessment of the river was done using microbiological parameters (HPC, TC, FC, and FS) for 7 months in Sooc Bridge and Carpenter’s Bridge using bottom sediments for samples. Sediment was used because bacteria accumulate over time in the sediment due to high amounts of organic and inorganic matter in the substrate and this leads to better survivability of bacteria. The values obtained from microbiological parameters investigated are generally higher in Sooc Bridge. HPC results show that the higher mean value was in Sooc Bridge(7.43 x 108 CFU/lOOg). The levels of TC on both sampling sites ranged from 9.5 x 102to 2.40 x 104 CFU/100g. For TC, Sooc has the higher mean (1.76 x 104 CFU/lOOg) for 7 months. FC values ranged from 3.0 x 101 to 2.4 x 104 CFU/lOOg. Sooc bridge has the higher mean(1.17 x 104 CFU/lOOg) between the 2 sites. Lastly, the FS value range from 2.9 x 103 to 2.4 x 104 CFU/lOOg. Also, Sooc Bridge has the higher mean value (1.58 x 104 CFU/lOOg). There are no significant differences on the microbiological parameters used in each site. The FC/FS ratio revealed that mixed fecal materials contaminate the river. Among the four pathogenic bacteria detected, Salmonella spp. was the most abundant for 7 months (63-65%) and E. coli was the least abundant(>l%). Based on the criteria on sediment quality evaluation, there is little to moderate loading of organic and inorganic matter.
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    Microbiological water analysis of selected point sources in Iloilo City
    Alama, Jannie Mae G.; Calitis, Ma. Socorro Fatima P.; Cervantes, Ria Angela E.; Garcia, Marian Aisa C.; Otico, Pelita A.; Pacheco, Donna Ginn G.; Taquiso, Jezreel l. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2006-04)
    The recreational use of the Iloilo River according to the Iloilo River Master Plan entails various studies to support it being safe for swimming and other activities. The Department of Environment and Natural Resources (DENR) Region VI has done very few studies to determine this particular aspect of the Iloilo River. Thus, the present study was conducted to determine the bacteriologic content of water in selected point sources in Iloilo River, Iloilo City and the possible correlations to the incidence of health risks. Specifically the study aims to: (1) determine significant differences in the physico-chemical characteristics of water samples from each point source; (2) to determine the total and fecal coliform counts number for each water sample from the selected point sources; (2) detect the presence or absence of E. coli, Salmonella spp., and culturable Vibrios in water in selected point sources; (3) describe the incidence of health risks; and (4) correlate the heterotrophic plate count for total coliforms, Most Probable Number for Fecal Coliforms and the number of symptomatic respondents. The results of the study showed that the total coliform count for Point source A (HPC=6000/l00 ml) is higher compared to the standard set by the DENR (5000/100 ml) while Point source B and C are within the range. The concentrations of E. coli, in water samples A, B, and C (2400 MPN/100 ml sample) were found to be higher than the DENR standard for fecal coliforms for Class C Waters - fresh water, (200 MPN/lOOml sample). Although results indicate that the river water has high fecal contamination, there were no significant correlations between heterotrophic plate counts, Most Probable Number for Fecal Coliforms and the number of symptomatic respondents for each point source.