Undergraduate Special Problem
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Item Antimicrobial activity of macrofungal basidiomycetous mycelia against Vibrio spp.Bagacay, Jann Felnesh Exe V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2023-06)Aquaculture is vital for global food security, producing 50% of the world’s fish. However, infectious diseases caused by fish pathogens like Vibrio species can lead to significant production losses. To address this issue, antimicrobial agents are commonly used. Macrofungal fruiting bodies and mycelia produce bioactive compounds with antimicrobial properties. This study aims to: (a) assess antimicrobial activity in isolated macrofungal samples against the fish bacterial pathogens Vibrio parahaemolyticus and Vibrio cholerae, (b) compare two methods (agar plug and crude extracts) for antimicrobial activity, (c) determine the minimum inhibitory concentration (MIC) using agar well diffusion and the agar plug methods, and (d) identify the macrofungal isolate with the highest antimicrobial activity. Ten morphospecies were collected from the University of the Philippines Visayas Miagao Campus. The samples were evaluated using the agar plug and agar well diffusion methods. The results showed antimicrobial activity against Vibrio cholerae and Vibrio parahaemolyticus. The activity index ranged from 0 to 0.83 and 0.22 to 0.78 for agar wells, and 0 to 1.00 and 0.70 to 1.30 for agar plugs, against V cholerae and V. parahaemolyticus, respectively. Termitomyces globulus and Trametes sp. 1 exhibited the highest activity indices using the agar plug and agar well diffusion methods, respectively. Statistical analysis confirmed significant differences in antimicrobial activity against both test organisms. These findings demonstrate the potential of macrofungi as a valuable source of antimicrobial compounds for controlling fish pathogens in aquaculture. Further research could lead to the identification and utilization of these compounds to minimize production losses and improve disease management in aquaculture.Item Antibacterial activity of aqueous and methanol extracts of selected basidiomycetes from UPV, Miagao, Iloilo PhilippinesAmbubuyog, Marja Victoria U. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-03)The Basidiomycetes represent an enormous source for natural products with diverse chemical structures and wideranging antimicrobial activity. This study was done to investigate the antibacterial property of selected species of basidiomycetes aqueous and methanolic extracts against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Specifically, it aimed to (1) determine the antibacterial activity of the aqueous extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa based on the zone of inhibition; (2) determine the antibacterial activity of the methanolic extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa based on the zone of inhibition; and (3) compare the aqueous and methanolic extracts of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa based on the zone of inhibition. A total number of ten species were collected namely Auricularia sp., Schizophyllum sp., Trametes pubescens, Trametes versicolor, Trametes elegans, Ganoderma sp.(l), Ganoderma sp.(2), Stereum sp. and two unknowns. Dried samples were crushed and extracted with water and methanol. Antibacterial activity was determined using the Kirby-Bauer disc diffusion method. The results showed a negative to weak inhibition against the test microorganisms which is inconsistent with the results of previous studies. It could possibly be attributed to the preparation of the samples and the fruiting body maturity that influenced the antibacterial potential of the samples. It appears that fruiting bodies used in the present study may not be reliable sources of bioactive compounds for antibacterial activity.Item Species composition and distribution of mangrove soil fungi from Iloilo River, Iloilo City, PhilippinesAlmoros, Peter Paul C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)Fungi are very diverse and ubiquitous microorganisms. Fungal diversity is an important aspect of the ecosystem that needs to be studied. There have been many studies regarding biodiversity of fungi in terrestrial, marine and riverine environments, but studies regarding fungi found in mangrove soil still needs to be done. This study focused on determining the species composition, distribution and ecology of mangrove soil fungi in the Iloilo River, Iloilo City. Soil fungi were sampled from three areas in the river representing the downstream, midstream and upstream areas. Fungal isolates were identified based on morphological characteristics- cultural and microscopic features. There were 10 identified fungal species -Aspergillus cf. flavus, Aspergillus cf. niger, Aspergillus cf. ustus, Aspergillus cf. versicolor, Aspergillus sp. (1), Aspergillus sp. (2) and Aspergillus sp. (3), Geotrichum cf. candidum, Penicillium cf. citrinum and Penicillium sp. (1), while there were 2 unidentified species. Three of these species were very frequent across the sites -Aspergillus cf. flavus, Geotrichum cf. candidum and Penicillium cf. citrinum.Item Short-term impacts of oil spills on fungal population in a mangrove area in Batad, IloiloAlpas, Donna Bella C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)Oil spills cause extensive damage on the marine environment, ranging from obliteration of its flora and fauna to biomagnifications of toxic components of petroleum. The location of mangroves at the sea-land interface makes the ecosystem susceptible to disturbances such as oil spills. There is a considerable information on the impacts of oil spills on mangrove while impacts on microbial populations, especially fungi, are limited. This study therefore assessed the effect of oil on the diversity and distribution of fungi of oil-contaminated sites in Batad, Iloilo due to Power Barge oil spill last November 8, 2013. The mangrove surface sediments were collected on January 11, 2014 at oil-contaminated sites in Batad, Iloilo and uncontaminated site in Ajuy, Iloilo. Sample were processed based on Pepper and Gerba (2004). Colony forming units per gram of soil (CFU/g) was used to express yeasts and filamentous fungi. On PDA medium, more fungal isolates were isolated at oil-contaminated site compared with uncontaminated site at 14 and 12 species respectively, there was also a higher Shannon Index of Diversity at 2.4858 and 2.4582, respectively. Among the filamentous fungi, three genera were represented by Aspergillus. Penicillium, and Cladosporium. However, Aspergillus was more diverse with 10 species. Six species were found only at the oil-contaminated sites - Aspergillus sp 2. Aspergillus sp 1, Cladosporium cladosporioides, Penicillium sp 2, Penicillium sp 3 and Yeast sp 2 while four species are found in the unoiled site - Penicillium cf. lapidosum, Aspergillus sp 3, Aspergillus sp 4 Penicillium sp 1. Furthermore, there is a low index of similarity as shown by Jaccards coefficient of similarity (J=0.44) and Sorensen coefficient of similarity ( C=0.61). Fungal load was higher at oil-contaminated soils compared with uncontaminated soils at 1.3 x 10 - CFU/g and 6.0 x IO3 CFU/g, respectively. Bushnell Haas Agar yielded more fungi isolates isolated from oil-contaminated sites compared with uncontaminated site of 4 and 3 species respectively. Furthermore, a higher Shannon Index Diversity (H) was observed at oil-contaminated sites (1.3298) than those at uncontaminated site (0.6364) suggesting that there are more species of fungi capable to degrade the hydrocarbons and utilize it as a source of energy. The overall frequency of distribution of the major fungal genera in both oil-contaminated and uncontaminated sites showed that only Dueteromycota was isolated and dominated in both sites and in both media, the PDA and Bushnell Haas Agar. The major genera isolated in PDA medium included Aspergillus, Penicillium, Cladosporium, and yeast. In Bushnell Haas Agar, Aspergillus and yeast were the major genera isolated and are seen in both sites. The frequency of occurrence of various species in uncontaminated and oil-contaminated sites were not significantly different. The Deuteromycota were the dominant group that were isolated on all sites using two media. The overall result indicate the stimulating effect of oil are observed in the higher fungal load in oil- contaminated site.Item Endophytic fungi from Halimeda macroloba and Sargassum sp.in Nueva Valencia Nueva Valencia, Guimaras, PhilippinesAlemania, Darlene Kris V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)Endophytic fungi of marine algae in the Philippines have not been widely explored despite the fact that marine fungi associated with algae are the second most diverse. This study aimed to conduct a preliminary investigation on the occurrence of endophytic fungi from Halimeda macroloba and Sargassum sp. In this study fungal endophytes were isolated from Halimeda macroloba and Sargassum sp. collected from Nueva Valencia, Guimaras. Broad-leaf macroalagal samples were surface sterilized and cultured on PDA seawater media at 37°C. Isolated fungal endophytes were identified through colony and microscopic characteristics. Twenty one distinct endophytic fungi were isolated, 17 of which were identified up to the genus level with the most endophytes belonging to the genus Aspergillus. Fungal endophytes obtained were mostly ascomycetes specifically Aspergillus sp., one hyphomycete and three unidentified fungi with sterile mycelia were present. The brown algae, Sargassum sp., had a higher diversity compared to the green algae Halimeda macroloba in spite of having a lower isolation frequency. In terms of similarity, the two algal hosts had a low index of similarity with J= 10%. This study contributes to the growing data regarding diversity and abundance of fungal endophyte species from marine macroalgae in the Philippines. This is the first study conducted on fungal endophyte assemblages of algae species in Western Visayas.Item Living above and belowground biomass and carbon stock assessment of station 1, Taklong Island, Taklong Island National Marine Reserve, Guimaras, PhilippinesAlegado, Alexis Jude T. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)The Taklong National Marine Reserve is one of the marine protected sites in the country with a good cover of mangroves. This study assessed the species composition, diversity, aboveground biomass and the estimated carbon of the natural mangrove forest found in Taklong Island Station 1. Barangay Lapaz. Nueva Valencia. Guimaras. The study aimed to: a.) map out the area of the selected mangrove patches, b.) identify the species composition and diversity of the sampling site, c.) quantify the aboveground and belowground biomass, carbon stock of live vegetations, and d.) to convert the carbon stock to carbon dioxide equivalence (CO₂e) of the selected station. This study is only limited to the estimation of living aboveground, belowground and the carbon stock using the published allometric equations. Only 7 species of mangroves were found in the site belonging to 3 families. The mean stand basal area was 21.47 m2 ha-1. The species diversity is low at 0.23 due to low speciation of species of mangroves on the site. The total aboveground biomass was 506.89 Mg ha-1 and carbon dioxide emission potential (CO₂e) of 2,498.15Mg CO₂e. This shows that mangrove in Station 1 of Taklong Island has stored a considerable amount of carbon overtime. Hence, there must be a proper management and protection of this ecosystem.Item Community structure, biomass production and carbon stock assessment of mangroves in Barangay Ambolong, Batan, AklanAgregado, Leizel B. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)Mangroves are evergreen shrubs and trees of several unrelated families that thrive within intertidal zones of sheltered flats, coves, bays, and estuarine regions extending inland along streams and river where water is primarily brackish. Continued threats to their existence call for their protection and conservation. This study was conducted to assess the community structure of mangroves in Brgy. Ambolong, Batan, Aklan by 1). measuring physico-chemical parameters (salinity, temperature, pH) 2). describing the community structure in terms of and in terms of a) basal area, b) stems per hectare, c) relative density, d) relative frequency, e) relative dominance, f) importance value, g) Index of Diversity, h) seedling and sapling density, and i) zonation pattern; 3). to assess the bidmass production and carbon stock. This study was done to provide preliminary information regarding the current status of mangroves in the area. Three (3) transect lines, each containing three (3) plots, were established in the area. Twenty (20) mangrove species belonging to eleven (11) families were identified: Acanthaceae - Acanthus ebreatus, Acanthus volubilis; Arecaceae - Nypa fruticans; Avicenniaceae - Avicennia alba, Avicennia marina, Avicennia officinalis, Avicennia rumphiana; Bombaceae — Camptostemon philippinensis; Euphorbiaceae - Excoecaria agallocha; Meliaceae — Xylocarpus grioanatum, Xylocarpus moluccensis; Myrsinaceae — Aegiceras corniculatum; Myrtaceae - Osbornia octodonta; Rhizophoraceae - Bruguiera cylindrica, Ceriops decandra, Rhizophora apiculata, Rhizophora mucronata, Rhizophora stylosa; Rubiaceae - Scyphiphora hydrophyllacea; Sonneratiaceae - Sonneratia alba. Water salinity in the study area ranged from 27-30 ppt; soil temperature ranged from 28°C to 30°C. Avicennia marina had the highest values for basal area (4.22 m2/ha), stems per hectare (15,700 stems per hectare), relative density (63.56), relative frequency (63.56%), relative dominance (48.10%), and was the most important species with the importance value of 175. Rhizophora stylosa had the lowest values for basal area (0.002 m2/ha), stems per hectare (100 stems/ha), relative density (0.40 trees/ha), relative frequency (0.4%), relative dominance (0.23%), and was the least important species, having the importance value of 0.832. Rhizophora apiculata and Avicennia officinalis, together with Rhizophora stylosa, had the lowest value for stems per hectare (100 stems/ha), relative density (0.40 trees/ha), and relative frequency (0.4%). Seedling density was highest for Avicennia alba(187 seedlings/ha) and lowest for Bruguiera cylindrica (1 seedling/ha). Sapling density was highest for Avicennia marina (54 saplings/ha) and lowest for Excoecaria agallocha (1 sapling/ha). The Index of Diversity was H=0.598. The highest recorded litter was 336.6 g in December 19, 2012-January 2, 2013 while the lowest was recorded at 18.2 grams in December 5-December 29, 2012. Highest biomass production was that of Avicennia marina (962.94 tons/ha), while the lowest was Avicennia officinalis (0.009 tons/ha). Avicennia marina had the highest carbon stock value of 433.32 tons/ha; the lowest was that of Avicennia officinalis (0.004 tons/ha).Item Assessment of damage to mangroves in Taklong Island National Marine Reserve, Guimaras, Philippines: 10 years after the 2006 oil spillAmistas, Jhon Robert (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2017-06)Approximately 2 million liters of bunker C was spilled by M/T Solar I off the coast of southern Guimaras on August 11, 2006. This is the worst oil spill in the country to date that affected the mangroves within Taklong Island National Marine Reserve, Nueva Valencia, Guimaras. Rapid assessment of impacts on the mangroves was done one month after the spill and long-term monitoring was done from 2007-2014. This study was conducted to determine the mangrove status 10 years after the spill in terms of community structure. Specifically, it determined a) species composition, b) stand basal area, c) stand height, d) relative density, e) relative dominance, e) relative frequency, f) importance value, g) index of diversity, h) species evenness, i) density of seedling, saplings, and trees. In addition, leaf area was also determined as an indicator of the health conditions of the species present. The same five stations in earlier studies were used, namely Bagaman, Pototan, Taklong Island (oiled), Tandog and Taklong Island (unoiled), Nueva Valencia, and Guimaras. Results showed that the community structure showed an improvement, especially in stand, basal area, wilding, and tree density while no change in terms of species diversity due to the absence of new species. The leaf areas of mangroves in Bagatnan indicated some signs of stress but showed an improvement since 2006. In general, the oil-affected mangroves in TINMR have shown signs of recovery. However, it is recommended that additional monitoring activities be conducted.Item Inhibition of bacterial Biofilm formation by selected wild Macrofungi from Miagao, IloiloAnacita, Beatrice L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2023-07)Biofilms are complex bacterial communities with high-stress tolerance and antimicrobial resistance, posing a global health concern. Mushrooms are known for their bioactive compounds, including antimicrobial properties, and may offer a solution for inhibiting biofilm formation. This study investigates the biofilm inhibition activity of wild macrofungi collected in Miagao, Iloilo. Five basidiomycetous macrofungi—Panaeolus sp., Chlorophyllum hortense, Lentinus concavus, Hexagonia hydnoides, and Daedalea sp.—were characterized. Bacterial strains were treated with equal concentrations of ethanolic mycelial extracts from the macrofungi samples and evaluated for adherence and inhibition percentage using a microtiter plate assay at 37°C for 72 hours. The results showed strong inhibition (>50%) of all macrofungi extracts against S. aureus. Hexagonia hydnoides exhibited the highest inhibition against both S. aureus (84.3%) and E. coli (14.1%). Chlorophyllum hortense, Lentinus concavus, Daedalea sp., and Panaeolus sp. demonstrated 83.7%, 82.4%, 81.25%, and 80% inhibition of S. aureus biofilm formation, respectively. However, the inhibition observed with all extracts against E. coli was not statistically significant (all <15%). The significant findings, particularly in inhibiting S. aureus biofilm production, indicate the presence of biological activity in the tested macrofungal extracts. These findings suggest their potential usefulness in the search for antibiofilm treatments