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    Spectroscopic investigation of Pyruvate formate lyase-activating enzyme: A look into EPR, ENDOR and Mössabuer spectroscopy
    Ortillo, Danilo O.; Broderick, Joan B. (Maxwell Science Publications, 2014)
    Electron Paramagnetic Resonance (EPR) and Electron Nuclear Double Resonance (ENDOR) spectroscopies are extremely powerful and versatile methods for the characterization of paramagnetic systems in biology, chemistry and physics. For iron centers in the radical SAM enzymes however, Mössbauer spectroscopy has proven to be both powerful and useful as a complementary spectroscopic technique in determining not just the oxidation states but also the type of iron species present in the catalytic center. The cluster content of the radical SAM protein, Pyruvate Formate-Lyase-Activating Enzyme (PFL-AE), was characterized using EPR and Mössbauer techniques while additional ENDOR analysis helped determine the novel interaction of the co-substrate, S-Adenosylmethionine (SAM or AdoMet) with the Fe-S cluster of PFL-AE. The anchoring role of the Fe-S cluster to the co-substrate derived from the spectroscopic data supports the mechanism where a SAM-based radical species is involved during catalysis. © Maxwell Scientific Organization, 2014.
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    Effects of Vinegars and Sodium Acetate on the Growth Performance of Pacific White Shrimp, Penaeus vannamei
    Jamis, Jhumar O.; Tumbokon, Barry Leonard M.; Caigoy, Jant Cres C.; Bunda, Marj Gem B.; Serrano, Augusto E. (Israeli Journal of Aquaculture - Bamidgeh, 2018)
    Vinegars and their salts have the potential to act as growth promoters and prophylactics against bacterial pathogens. This study aims to evaluate the effects of various vinegars and sodium acetate on the growth performance of white shrimp Penaeus vannamei. Groups of shrimps were fed diets containing 2% of either apple cider vinegar (ACV), coconut sap vinegar (CSV), sugar cane vinegar (CaV), or sodium acetate, and a diet with no vinegar (i.e. a control diet). Total acidity data of the diet showed that only the sodium acetate diet resulted in the highest total acidity after 60 min immersion in salt water (20 ppt). Attractability tests using customized repartitioned aquaria showed that the CSV diet attracted the highest significant percentage of shrimps after 10 min of feed placement in the feeding chamber. In the feeding trial that lasted for 60 days, results showed that the ACV and CSV groups of shrimps consumed significantly more feed than the other groups. All vinegar groups exhibited significantly higher final average body weight, weight gain, and specific growth, rate than either the control or sodium acetate group. The CSV group exhibited the significantly best feed conversion ratio and protein efficiency ratio. Survival was statistically similar among all groups. Conclusion: the CSV group exhibited the significantly best growth performance and efficiency while both the control and sodium acetate groups exhibited the poorest. © 2018, Israeli Journal of Aquaculture - Bamidgeh. All rights reserved.