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Masters Theses

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/12

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Degree: search.filters.degree.Master of Science in Fisheries (Fish Processing Technology)Discipline: search.filters.discipline.Fisheries

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    Antioxidative activities and lactic acid bacteria composition of fermented frigate tuna at different salt-fish ratio
    Ortizo, Rhessa Grace G. (University of the Philippines Visayas, 2019-06)
    The antioxidative activities and lactic acid bacteria (LAB) composition of fermented frigate tuna (Auxis thazard), locally known as tinabal, at different salt-fish ratios were investigated. Fresh frigate tuna were degutted and cleaned before the addition of salt at different salt-fish ratio (w/w), (1:3), (1:4), (1:5), (1:6), and then fermented at ambient temperature. Samples were taken on day 0, 1, 4, and 7 for the pH determination, total titratable acidity measurement, and the different antioxidant activity assays that include testing for radical scavenging activity of ABTS+, ferric reducing power, hydrogen peroxide and singlet oxygen radicals. Isolation of lactic acid bacteria (LAB) for bacterial identification was also conducted as well as the measurement of the degree of hydrolysis for peptide profile analysis of fermented product. Results show that the LAB viable count in the fermented samples increased, and the pH levels decreased with increasing % titratable acidity during the 7 days of fermentation. Isolated LAB and LAB-like species with gram positive, catalase negative, and non-spore forming properties were presumptively identified as Lactobacillus casei, Lactobacillus fermenti, Lactobacillus delbrueckii subsp. delbrueckii, Pediococcus pentosaceus, Leuconostoc spp., Enterococcus faecalis and Streptococcus spp. In this study, the free amino acids due to hydrolysis increased with fermentation time for the different salt-fish ratios and DH was influenced by the length of fermentation. The ethanolic extracts from F3 (1:6) sample showed strong antioxidative activities against ABTS+ radical, while sample Fl (1:4) showed high reduction of ferric ions, and sample F2 (1:5) exhibited high antioxidative activities against 1O2 radical which is the major cause of damage to bioactive compounds that leads to oxidative stress in fermented products. Results further revealed that tinabal could be a potential source of natural antioxidants for industrial uses. In addition, presence of important LABs in F2 (1:5) indicated that this fermented product could serve as a good source of LAB for applications to other fermented products as starters for improved product quality. In particular, tinabal fermented with F2 salt-fish ratio (1:5) has potential to be a functional food product that could benefit the consumers.
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    Nutritional value of fish silage, with Gracilaria Sp. and Abalone Viscera as poultry feed
    Patadjai, Andi Besse (University of the Philippines Visayas, 2004-11)
    Study on fish silage prepared with , with and without abalone viscera was conducted to evaluate the rate of hydrolysis during the ensiling process. The fish-Gracillaria sp.-abalone viscera silage was further subjected to nutritional value test as poultry feed. Three silage were prepared using 5% propionic acid and formic acid (ratio 1:1) to contain: a) 100% fish by catch (Silage F), b) 70% fish by catch and 30% Gracilaria sp. (Silage FG) and c) 70% fish by catch, 25% Gracilaria sp. and 5% abalone viscera (Silage FGA). These silage were stored for 15 days and stirred daily. Chemical components such as pH, amino nitrogen, polypeptide, and monosaccharide content were determined at regular intervals within the 15-day ensiling period. Isonitrogenous feeds were then formulated to contain fish-Gracillaria sp.- abalone viscera silage Feed (II) and fish silage (Feed III). Nutritional value of these diet (i.e. Feed II and Feed III) were compared with that of commercial feed (Feed I) which was used as the control, based on the growth response of chickens. Results showed that rate of decrease in pH value was sufficiently rapid to stabilize the three silage. Silage F showed significantly higher pH value throughout the ensiling period, compared with Silage FG and Silage FGA. No significant difference (P<0.05) was, however, found between Silage FG and Silage FGA. Results can be attributed to incorporation of Gracilaria sp. in silage production, which provided favorable condition to decrease the pH value. Amino nitrogen content of all silage increased with ensiling time. Silage F exhibited the highest amino nitrogen content during 15 days of ensiling, followed by that of Silage FGA. Although the amino nitrogen content of Silage FGA was higher than that of Silage FG, significant differences (P<0.05) were noted only after 12 days of ensiling. Polypeptide content of all silage decreased after 3 days of ensiling. Silage FGA exhibited the highest polypeptide content after 9 days of ensiling. Monosaccharide content of all silage fluctuated during the ensiling period. However, the highest value was noted with Silage FGA, followed by Silage FG on the 3rd day of ensiling. This could be attributed to the highest polysaccharide hydrolysis rate exhibited by Silage FGA throughout the ensiling period. The best feed conversion ratio (FCR) and protein efficiency ratio (PER) were obtained with chickens given feed containing fish-Gracilaria sp. -abalone viscera silage (Feed II) during the 3-week feeding time. Body weights of chickens given Feed II were significantly higher than those given Feed III until the 3rd week of feeding. Reduction of fish content by as much as 30% and its substitution with 25% of Gracilaria and 5% of abalone viscera in silage (Feed II) gave the same effect on growth rate of chickens as that of silage made up of 100% fish (Feed III), and commercial feed (Feed I) without any mineral deficiency symptoms (e.g. slipped tendon or leg weakness and abnormal feather growth). Fish-Gracilaria .sp.-abalone viscera silage could be used as an ingredient for the production of an acceptable/palatable and nutritious feed for poultry.