UPV Digital RepositoryUPV-DRUniversity of the Philippines Visayas
 

Masters Theses

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/12

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Committee Member: search.filters.committeemember.Cainglet, Rica P.

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    Does a coral reef enhance the productivity of its surrounding waters?
    Peñafiel, Christen P. (University of the Philippines Visayas, 2019-06)
    Coral reefs have long been assumed and continually claimed to be origins of very high productivity, but how they attract organisms from and export nutrients to their neighboring waters remains largely unknown. Specifically, this study aimed at determining the space-time dynamics of nutrients and biomass redistribution around Hibotkan Rock Sanctuary (HRS), Banate, Iloilo, a sanctuary reef established by Municipal Fisheries Ordinance No. 12 covering an area of 25 ha. Twelve (12) sampling stations were established for space-time nutrient profiling, phytoplankton count, and supplementary measurements of water parameters, and four (4) stations for fish (biomass) visual survey. Results were analyzed using spatio-temporal plots and the Pearson correlation matrix (5% significant level). Nitrite was observed to be limiting, while nitrate was the most abundant in the waters of HRS. The predominant temporal variations of nutrients followed the flood tide (FT) and ebb tide (ET) cycles. In general, the tidal current direction appeared to control the redistribution of the nutrients (NO3-, NO2-, PO43-, and SiO44-). This study further revealed that stations of high nutrient concentration usually coincided with low levels of dissolved oxygen (DO). Species of fish observed were all indigenous and well-known inhabitants of the coral reefs. Furthermore, no other families of fishes (such as those of commercial value) were caught on record. Overall, there was no clear indication of import or export of living biomass in the HRS area. It can be inferred from the results that the fabled high productivity of coral reefs has no clear scientific basis; consequently, the Marine Protected Areas (MPAs) concept also appears to have no solid foundation.
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    Isolation of Hyaluronic acid from yellowfin tuna (Thunnus albacares) eyeball
    Sumogod, Angeleca A. (College of Fisheries and Ocean Sciences, University of the Philippines Visayas, 2019-06)
    Hyaluronic acid (HA) is commonly extracted from terrestrial and bacterial sources. However, due to the risk associated with either animal- and/or bacterial- derived contaminants and the laborious production processes that tend to compromise the quality of HA, research studies have recently shifted to the exploration of the presence of this polymer in marine resources and the enhancement of the protocols for HA production. Hence, this study focused on the isolation of HA from yellowfin tuna eyeballs. Results of the study showed that the optimum conditions for the isolation of HA from yellowfin tuna eyeballs are as follows: pretreatment of the raw material (acetone, formaldehyde and sodium acetate solution) prior to water extraction and incubation of the mixture for 24 h; tissue hydrolysis for a maximum of 6 h; a 3% CPC: 3M NaCl concentration for recovery and fractionation; and the use of 1:3 v/v supernatant: ethanol ratio for alcoholic precipitation. These results are expected to help maximize the benefits of the utilization of the tuna processing wastes in the country. Likewise, these findings may open a new opportunity for more cost-effective production of a valuable bioactive compound from marine source.