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Masters Theses

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    The occurrence, distribution and abundance of larval stages of tunas in Ragay Gulf, Burias Pass, Ticao Pass and waters north of Samar Sea
    Abuso, Zenaida V. (University of the Philippines Visayas, 1988-03)
    A total of 146 tuna larvae representing a 0.004% share in the total fish larvae catch was collected from Ragay Gulf, Burias Pass, Ticao Pass and waters north of Samar Sea during the sampling period from November 1981 to December 1982. Seven species of tunas were identified and described to species level. Euthynnus affinis. and Thunnus obesus. comprised a highest relative abundance of 24.05% in the larval tuna collection. Auxis thazard and A. rochei were secondly abundant with 16.0% and 14.0% share, respectively. Thunnus albacares, Thunnus alalunga and Katsuwonus pelamis were relatively rare with only 5, 4 and 2,0% share, respectively. The highest abundance of the larvae were observed mainly irr Ragay Gulf in May where warmer temperatures ranging from 26.56°C to 28.9T°C and salinity values from 33.97°/oo to 34.42°/oo prevailed.
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    Isolation of Hyaluronic acid from yellowfin tuna (Thunnus albacares) eyeball
    Sumogod, Angeleca A. (College of Fisheries and Ocean Sciences, University of the Philippines Visayas, 2019-06)
    Hyaluronic acid (HA) is commonly extracted from terrestrial and bacterial sources. However, due to the risk associated with either animal- and/or bacterial- derived contaminants and the laborious production processes that tend to compromise the quality of HA, research studies have recently shifted to the exploration of the presence of this polymer in marine resources and the enhancement of the protocols for HA production. Hence, this study focused on the isolation of HA from yellowfin tuna eyeballs. Results of the study showed that the optimum conditions for the isolation of HA from yellowfin tuna eyeballs are as follows: pretreatment of the raw material (acetone, formaldehyde and sodium acetate solution) prior to water extraction and incubation of the mixture for 24 h; tissue hydrolysis for a maximum of 6 h; a 3% CPC: 3M NaCl concentration for recovery and fractionation; and the use of 1:3 v/v supernatant: ethanol ratio for alcoholic precipitation. These results are expected to help maximize the benefits of the utilization of the tuna processing wastes in the country. Likewise, these findings may open a new opportunity for more cost-effective production of a valuable bioactive compound from marine source.