Geduspan, Jane S.Buendia, Jeremiah C.2026-07-211994-03Buendia, J. C. (1994). <i>A preliminary study on the freezing tolerance of milkfish (Chanos chanos, Forskal) embryos</i> [Undergraduate thesis, University of the Philippines Visayas]. UPV Institutional Repository. https://hdl.handle.net/20.500.14583/761https://hdl.handle.net/20.500.14583/761Milkfish embryos of blastula and C-shaped stages were tested for cryoprotectant toxicity using DMSO and glycerol at 10%, 12% and 15% concentrations. Survival and hatching as well as the effects of freezing were observed. Glycerol was more toxic than DMSO. Freezing at 4°C resulted in the decrease of hatching, and ice crystal formation killed the embryos during the process of freezing to -80°C. C-shaped embryos had a higher survival and hatching than the blastula stage embryos in the toxicity and cold shock tests.viii, 37 p.MilkfishFish embryosCryopreservationDimethyl sulfoxide (DMSO)AquacultureLG 993.5 1994 B5 B94Thesismilkfishanimal embryoscold toleranceegg hatchabilityaquacultureSDG 2 - Zero hungerSDG 9 - Industry, innovation and infrastructureSDG 14 - Life below water