Ortillo, Danilo O.Araniador, Glaicyl Dame Ann B.2025-07-142019-06Araniador, G. D. A. B. (2019). Optimization of parameters for the Fe-humic acid complex formation using fluorescence spectroscopy [Undergraduate thesis, University of the Philippines Visayas]. UPV Institutional Repository. https://hdl.handle.net/20.500.14583/316https://hdl.handle.net/20.500.14583/316Humic acid was extracted from an artesian well located at Sta. Barbara, Iloilo. It was black in color and had a powdery texture after oven-drying. Humic acid samples were used to prepare complexes with Fe3+ ions to determine the ideal conditions to maximize the binding of the metal ion. Using the fluorescence data of the ligand as a comparative measure to confirm complex formation, varying concentration and pH conditions were used in preparing Fe-HA complexes. The excitation spectra of the different concentration ratios prepared showed that pH 6 favored the complex formation. The emission spectra showed that pH 6 exhibited high fluorescence signals. The EEMs of different concentration ratios at pH 6 showed that it is the optimal range for complex formation. In pH 8 and pH 10, no consistent pattern in their EEMs can be observed. This indicates that alkaline conditions, complex formation is not favored.xiv, 60 p.Fe-HA complexFluorescenceEEMsExcitationEmissionOptimizationLG 993.5 2019 C4 A75Thesishumic acidsmetalsalkalinespectral analysisSDG 6 - Clean water and sanitation